Variation in the diversity and richness of parasitoid wasps based on sampling effort.

Darren F. Ward,Thomas E. Saunders

doi:10.7717/peerj.4642

Abstract

Parasitoid wasps are a mega-diverse, ecologically dominant, but poorly studied component of global biodiversity. In order to maximise the efficiency and reduce the cost of their collection, the application of optimal sampling techniques is necessary. Two sites in Auckland, New Zealand were sampled intensively to determine the relationship between sampling effort and observed species richness of parasitoid wasps from the family Ichneumonidae. Twenty traps were deployed at each site at three different times over the austral summer period, resulting in a total sampling effort of 840 Malaise-trap-days. Rarefaction techniques and non-parametric estimators were used to predict species richness and to evaluate the variation and completeness of sampling. Despite an intensive Malaise-trapping regime over the summer period, no asymptote of species richness was reached. At best, sampling captured two-thirds of parasitoid wasp species present. The estimated total number of species present depended on the month of sampling and the statistical estimator used. Consequently, the use of fewer traps would have caught only a small proportion of all species (one trap 7–21%; two traps 13–32%), and many traps contributed little to the overall number of individuals caught. However, variation in the catch of individual Malaise traps was not explained by seasonal turnover of species, vegetation or environmental conditions surrounding the trap, or distance of traps to one another. Overall the results demonstrate that even with an intense sampling effort the community is incompletely sampled. The use of only a few traps and/or for very short periods severely limits the estimates of richness because (i) fewer individuals are caught leading to a greater number of singletons; and (ii) the considerable variation of individual traps means some traps will contribute few or no individuals. Understanding how sampling effort affects the richness and diversity of parasitoid wasps is a useful foundation for future studies.

Highlights

Invertebrates comprise the vast majority of global species richness, drive wide-ranging ecological processes, and provide ecosystem services essential for human prosperity (Mora et al, 2011; Chapin et al, 2000; Potts et al, 2010)
Parasitoid diversity A total sampling effort of 840 Malaise-trap-days at both sites over a period of three months resulted in the capture of 61 morphospecies, from 568 individuals
Despite the intensive sampling effort, only 13 species comprised more than 10 individuals, while 40% of species were singletons (n = 24) and 16% were doubletons (n = 10)

Summary

Introduction

Invertebrates comprise the vast majority of global species richness, drive wide-ranging ecological processes, and provide ecosystem services essential for human prosperity (Mora et al, 2011; Chapin et al, 2000; Potts et al, 2010). Despite their obvious importance, there are numerous impediments to a wider appreciation of their diversity, ecology, How to cite this article Saunders and Ward (2018), Variation in the diversity and richness of parasitoid wasps based on sampling effort. Parasitoids can be highly prone to extinction because of their reliance on hosts in lower trophic levels (Shaw & Hochberg, 2001)

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