Abstract
Fatty acid binding proteins (FABPs) bind long-chain fatty acids and are involved in their intracellular transport. Of the known bovine FABP genes, FABP4 has been mapped to a region on chromosome 14 that contains quantitative trait loci for milk traits. This study investigated the association of FABP4 haplotypes with milk production traits in 719 Holstein-Friesian × Jersey cows. Polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis of a variable region of the gene revealed three haplotypes (A, B and C). Five single nucleotide polymorphisms (SNPs) were identified: two in exon 3 and three in intron 3. A was associated (P = 0.032) with increased milk protein percentage (present: 4.00 ± 0.02%; absent: 3.95 ± 0.02%) and B was associated (P = 0.009) with increased milk yield (present: 23.81 ± 0.23 kg/d; absent: 23.06 ± 0.21 kg/d), but tended to be associated with a decrease in protein percentage and an increase in protein yield. Cows with genotypes AA, AB and AC produced less milk, but with a higher protein percentage than BC cows. This suggest that FABP4 affects milk yield and milk protein content, both economically important traits, and that further study of this gene is warranted.
Highlights
Fatty acid binding proteins (FABPs) bind long-chain fatty acids and are involved in their intracellular transport
Fatty acid binding proteins (FABPs) bind long-chain fatty acids and transport them within cells. They have been implicated in lipid metabolism, the expression of fatty acid-responsive genes and the maintenance of cell membrane fatty acid levels[1,2]
In the bovine mammary gland, the most abundant FABP mRNAs are for FABP3, FABP4, and FABP5; and for all, gene expression is greatly up-regulated during lactation[4]
Summary
Fatty acid binding proteins (FABPs) bind long-chain fatty acids and are involved in their intracellular transport. Of the known bovine FABP genes, FABP4 has been mapped to a region on chromosome 14 that contains quantitative trait loci for milk traits. Fatty acid binding proteins (FABPs) bind long-chain fatty acids and transport them within cells They have been implicated in lipid metabolism, the expression of fatty acid-responsive genes and the maintenance of cell membrane fatty acid levels[1,2]. FABP4 has been mapped to BTA145 in a location (46,833,665-46,838,053) that is rich in quantitative trait loci (QTL) for milk production traits[6,7]. This suggests it may be a candidate gene or marker for milk production. Eight single nucleotide polymorphisms (SNPs) have been validated in beef cattle, and of these, five SNPs (one non-synonymous SNP, one splice-site SNP and three intronic SNPs) are located in a small exon 3-intron 3 region[11] that would be suitable for polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis analysis
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