Variation in structure of proteins by adjusting reactive oxygen and nitrogen species generated from dielectric barrier discharge jet

Ji Hoon Park,Minsup Kim,Eun Ha Choi,Art. E. Cho,Masaharu Shiratani,Pankaj Attri

doi:10.1038/srep35883

Ji Hoon Park, Minsup Kim + Show 4 more

Open Access

https://doi.org/10.1038/srep35883

Copy DOI

Abstract

Over the last few years, the variation in liquid chemistry due to the development of radicals generated by cold atmospheric plasma (CAP) has played an important role in plasma medicine. CAP direct treatment or CAP activated media treatment in cancer cells shows promising anticancer activity for both in vivo and in vitro studies. However, the anticancer activity or antimicrobial activity varies between plasma devices due to the different abilities among plasma devices to generate the reactive oxygen and nitrogen species (RONS) at different ratios and in different concentrations. While the generation of RONS depends on many factors, the feeding gas plays the most important role among the factors. Hence, in this study we used different compositions of feeding gas while fixing all other plasma characteristics. We used Ar, Ar-O2 (at different ratios), and Ar-N2 (at different ratios) as the working gases for CAP and investigated the structural changes in proteins (Hemoglobin (Hb) and Myoglobin (Mb)). We then analyzed the influence of RONS generated in liquid on the conformations of proteins. Additionally, to determine the influence of H2O2 on the Hb and Mb structures, we used molecular dynamic simulation.

Highlights

Oxygen addition to Ar plasmas has been shown to have an important effect on plasma sterilization efficiencies, which contradicts the above study, in which the addition of oxygen decreases the efficiency[14]
Reactive oxygen and nitrogen (RONS) species produced by mixed gas plasmas in gas phase
To understand the reactive oxygen and nitrogen species (RONS) produced by Ar, Ar-O2 [addition at 0.2%, 0.3%, and 0.4% O2 in Ar gas], and Ar-N2 [addition at 0.2%, 0.3%. and 0.4% N2 in Ar] as feeding gas plasmas, we used optical emission spectroscopy (OES)

Summary

Introduction

Oxygen addition to Ar plasmas has been shown to have an important effect on plasma sterilization efficiencies, which contradicts the above study, in which the addition of oxygen decreases the efficiency[14]. The OH and O peaks decreased with the addition of N2 content compared with Ar plasma, but among the O2 mixtures, no change in intensity was observed.

Results

Conclusion