Variation in Salinity Tolerance in Sunflower (Helianthus annum L.)

Abstract

AbstractForty‐five accessions of sunflower collected from different countries were screened for salinity tolerance after 2 weeks growth in sand culture salinized with 150 meq l−1 of NaCl2+ CaCl2 (1:1 ratio equivalent wt. basis) in half strength Hoagland's nutrient solution. The results for plant biomass of 45 accessions show that there was considerable variation in salinity tolerance.In a further greenhouse experiment, the salinity tolerance of three tolerant (HO‐1, Predovik, Euroflor) and two sensitive (SMH‐24, 9UO‐985) lines (selected on the basis of their performance in the seedling experiment) was assessed at the adult stage to evaluate the consistency of salinity tolerance at different growth stages. All three salt tolerant accessions produced significantly greater plant biomass, seed yield and seed oil content than the salt sensitive accessions. The tolerant accessions accumulated less Cl− and more K+ in the leaves under saline conditions compared with the salt sensitive accessions. The salt tolerant accessions also maintained relatively high leaf K:Na ratio and K+ versus Na+ selectivity. Although statistically nonsignificant, all three tolerant accessions had greater soluble carbohydrates, soluble proteins, total free amino acids and proline in the leaves than the sensitive accessions. A field trial conducted in a salt‐affected field confirmed the greenhouse results of the selected accessions.This study shows that salinity tolerance of sunflower does not vary with stage of plant cycle, so selection for increased salt tolerance can be carried out at the initial growth stage. Secondly, it is found that there is great variation of salt tolerance in sunflower. Low uptake of Cl−, high uptake of K+, and maintenance of high K:Na ratios and K+ versus Na+ selectivity in the leaves and possibly the accumulation of organic osmotica such as soluble carbohydrates, soluble proteins, proline and free amino acids seem to be the important components of salt tolerance in sunflower.