Abstract
Isoelectrofocusing was used to identify isoenzyme polymorphism among strains of the entomogenous fungus, Beauveria bassiana (Balsamo) Vuillemin, attacking Sitona species in England, France, and Morocco. Protein extracts were prepared from monosporic isolates from 45 individual weevil cadavers. Buffer-soluble α-esterase variability was compared qualitatively among isolates and with a known reference strain using factorial correspondence analysis. The α-esterase system was found to be polymorphic, with 11 different profiles distinguished. All isolates from France north of the Loire Valley and England were monomorphic and similar to the reference strain originally isolated from Ostrinia nubilalis (Hübner) from northern France. The three isolates from France south of the Loire Valley and the 23 Moroccan isolates showed 10 different banding patterns. The heterogeneous southern populations of B. bassiana are considered to harbor a much wider adaptive capacity; thus they are richer sources of more effective pathotypes for possible redistribution elsewhere for enhanced biological control of Sitona spp. weevils. The isoelectrofocusing technique should prove useful in helping simplify selection of the more promising pathotypes.
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