Variation in flower biomass among nearby populations of Impatiens textori (Balsaminaceae): effects of population plant densities

Abstract

Although most of the previously detected variation in flower biomass was among populations that were far apart from each other, differences in population characteristics (population size, area, and plant density) may bring the variation in flower biomass to a more local scale. To examine the variation in flower biomass among nearby populations of Impatiens textori Miq. (Balsaminaceae), field studies were conducted on six natural populations located along a stream in Japan. We also examined the dependence of flower biomass and outcrossing rate on population characteristics, as well as the differences in plant size and pollinator behavior among populations. We conducted pollination experiments with potted plants, in which plant density and flower size were independently manipulated. Mean flower mass varied among populations, being negatively dependent on plant density. One-factor ANCOVA showed that both plant size and the other population-level factor affected flower biomass variation. Experiments with potted plant arrays showed that geitonogamous pollination more likely occurred in sparse populations, but in field studies, the population outcrossing rate was not significantly dependent on plant density of the population. Thus, the variation in flower biomass cannot be fully explained by these commonly considered factors. Our results show that the flower biomass of populations may evolve locally in response to plant density or other population characteristics.Key words: variation, flower biomass, population, Impatiens textori, plant density, local scale.