Abstract
Abstract Gametic differentiation in Chlamydomonas reinhardti is induced by the removal of ammonium (the nitrogen source) from the culture medium. Some of the major routes of ammonium incorporation into amino acids were therefore studied. The presence of alanine dehydrogenase ( l -alanine:NAD oxidoreductase, EC 1.4.1.1) and glutamate dehydrogenase ( l -glutamate:NAD(P) oxidoreductase, EC 1.4.1.3) was demonstrated in C. reinhardti and C. moewusii . It was observed that the activity of both enzymes underwent reproducible variations during synchronous vegetative development. The changes in activity of alanine dehydrogenase were inversely related to the respiration rate and to the specific activity of glutamate dehydrogenase. On the other hand, the specific activity of alanine dehydrogenase was directly proportional to the amount of alanine synthesized from 14 CO 2 by photosynthesis in vivo . Upon the removal of ammonium ( i.e the induction of gametogenesis) the variations in the activity of alanine dehydrogenase observed in the normal life cycle were markedly affected.
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