Variant of rs11196205 in TCF7L2 gene may be associated with type 2 diabetes and impaired glucose regulation in Anhui, China

Abstract

Objective To determine whether the variant of rs11196205 in TCF7L2 gene is associated with type 2 diabetes and impaired glucose regulation in Anhui Chinese population. Methods In this case-control study, we chose patients with type 2 diabetes (T2DM group, n=300), and impaired glucose regulation (IGR group, n=300), and normal control subjects (NGT group, n=300) (all from the First Affiliated Hospital of Anhui Medical University), and collected clinical characteristics and blood, extracted DNA. PCR amplified fluorescence-labeled target gene, which then hybridized with probes on the surface of biochip, and got the scanning results. All the data was analyzed by one-way ANOVA and K-W tests, finally determine whether variant of rs11196205 related to type 2 diabetes and impaired glucose regulation. Results The frequencies of rs11196205 C-allele was 21%, 19%, 11% in T2DM, IGR, and NGT group, respectively; the frequencies of genotype with risk allele (GC and CC) was 41%, 37%, 22% in T2DM, IGR, and NGT group, respectively. The C allele of rs11196205 had significantly increased the risk for type 2 diabetes(OR=2.08, 95%CI=1.51-2.86, χ2=20.68, P<0.05)and impaired glucose regulation(OR=1.84, 95%CI=1.33-2.54, χ2=13.71, P<0.05), or any of them(OR=1.96, 95%CI=1.46-2.61, χ2=21.18, P<0.05). Carriers of the C allele(GC and CC) had higher risk of having type 2 diabetes(OR=2.38, 95%CI=1.67-3.40, χ2=23.37, P<0.05)and impaired glucose regulation(OR=2.04, 95%CI=1.43-2.92, χ2=15.46, P<0.05), or any of them(OR=2.21, 95%CI=1.61-3.03, χ2=24.50, P<0.05). Conclusions The variant of rs11196205 in TCF7L2 gene may be associated with type 2 diabetes and impaired glucose regulation in a Chinese population. Key words: Polymorphism; Single nucleotide; Type 2 diabetes mellitus; Impaired glucose regulation; TCF7L2 gene