Abstract
Research has indicated higher concentrations of histamine and polyamine in endometrioid tissue in comparison with healthy tissue. The aim of this study was to evaluate changes in the expression patterns of messenger RNA (mRNAs) and microRNA (miRNAs) related to the histaminergic system in endometrial samples and whole blood in women with endometrioid endometrial cancer. The study group consisted of 30 women with endometrioid endometrial cancer qualified for hysterectomy (G1 well-differentiated, 15 cases; G2 moderately differentiated, 8 cases; and G3 poorly differentiated, 7 cases). The control group included 30 women with no neoplastic changes during routine gynecological examinations. The molecular analysis consisted of the microarray analysis of mRNAs and miRNAs related to the histaminergic system, reverse-transcription quantitative polymerase chain reaction (RTqPCR), and enzyme-linked immunosorbent assay (ELISA). Out of 65 mRNAs connected with the histaminergic system, 10 differentiate the samples of tissue and blood obtained from patients with endometrioid endometrial cancer in comparison with the control group (p < 0.05). mRNA histamine receptor 1,3 (HRH1, HRH3), and solute carrier family 22 member 3 (SLC23A2) differentiating samples of endometrioid endometrial cancer independent of either G or control. The highest probability of interaction, based on the target score miRDB, between the selected miRNAs and mRNAs was found for the hybrids hsa-miR-1-3p and endothelin 1 (END1), hsa-miR-27a-5β and SLC23A2. The selected mRNA and miRNA transcripts seem to be promising for molecularly targeted therapies in the context of endometrioid endometrial cancer.
Highlights
From among 22,277 mRNAs present on the HG-U133_A2 microarray slide, 65mRNAs are connected with the histaminergic system
The one-way ANOVA variance analysis conducted at the first stage showed that 10 from among the 65 mRNAs differentiate samples of tissues and blood obtained from patients with endometrioid endometrial cancer in comparison with the control (p < 0.05)
The indicated microarray profile of gene expression connected with the histaminergic system confirmed the same direction of the change in the expression of individual mRNAs, both in the biopsies of the endometrioid endometrial cancer as well as in the control
Summary
Endometrioid endometrial cancer (EC) is one of the most common malignant tumors in women in developed countries, and the incidence rate is constantly increasing. In the past 10 years, its frequency has increased by 1% each year and presently stands at 79 cases for every 100,000 women in Europe. The median age at the time of diagnosis is 62 years old. Endometrium endometrial cancer is generally detected at the early stages of advancement and commonly manifests itself as abnormal reproductive organ bleeding [1]
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