Abstract
Prunus necrotic ringspot virus (PNRSV) and Prune dwarf virus (PDV) are the most common pathogens in stone crop orchards. These diseases are easily transmitted with pollen and hence rapidly spread in orchards leading to stunting of trees, their increased susceptibility to abiotic stress factors and, eventually, to significant yield losses. In Ukraine, only monitoring studies on the spread of these viruses were conducted until now. However, phylogenetic comparison of Ukrainian isolates was lacking. In this work, total RNA was isolated from plant samples tested positive for PNRSV and PDV by ELISA. The part of viral CP gene sequences were amplified and sequenced with their subsequent phylogenetic analysis. It was determined that PNRSV isolates from Ukraine analyzed in this study belong to different groups – PV-96 (MT828889) and PV-32 (MT892676) with a maximum identity level of 100 % with known isolates from NCBI GenBank. PDV isolates (MT828888 and MT828887) showed high identity with each other (99.6 %), and Slovakian isolate from sweet cherry was shown as the most related to them with identity of 95.3 %.
Highlights
Sour cherry (Prunus cerasus L.) and sweet cherry (Prunus avium L.) are traditional fruit crops in Ukraine
These symptoms are characteristic of Prune dwarf virus (PDV) infection and have been repeatedly described by a number of authors (Massart et al, 2008; Smith et al, 1988; Sanchez et al, 2015; Kamenova et al, 2019)
The presence of both viruses in the plant was confirmed by ELISA and RT-PCR
Summary
Sour cherry (Prunus cerasus L.) and sweet cherry (Prunus avium L.) are traditional fruit crops in Ukraine. They have an important role in the structure of fruit and berry crop production. According to the preliminary data, prevalence of these viruses in mother plant orchard of cherries in Ukraine is 11.8 % for PNRSV and 8.7 % for PDV (Pavliuk et al, 2018). Both viruses belong to Ilarvirus genus, Bromoviridae family (Pallas et al, 2012). Infection by these viruses leads to a variety of symptoms, the manifestation of which depends on climatic conditions and the type of host plant (Kamenova et al, 2019)
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