Variability of secondary metabolism and morphogenesis of Ligustrum vulgare L. under different growing conditions in an urban environment

Abstract

Abstract Assessing the condition of plants in an urban landscaping is a necessary component of monitoring urban ecosystems. Studying how the sensitivity of plants to stressful conditions and the influence of many factors on their growth and development is important for determining the suitability of using plants for urban improvement. The purpose of this study was to identify the impact of stress factors on the growth and development of Ligustrum vulgare L. (commonly called privet). The research was conducted in the city of Kyiv, where the plants are affected by significant vehicle traffic, intense insolation or excessive shade, and mechanical traumatization of plants due to plant trimming. Shoots of Ligustrum vulgare L. of different ages and under different growing conditions were used for the analysis. The research used methods of morphometry, chromatographic profiling of secondary metabolites, and statistical data processing. To analyze the morphogenesis of annual shoots, the Gaussian model was used, which accurately (R2 > 0.90) describes their growth processes. The model coefficients were used for a comparative assessment of the growth dynamics of Ligustrum vulgare L. shoots depending on the growing conditions and plant care. Correlation analysis also allowed for the identification of potential connections between the growth model coefficients and certain phenolic synthesis products. It was found that the plant trimming leads to an increase in the length of the shoots and accelerated growth of the side shoots. The obtained results may indicate that phenolic compounds, in particular chlorogenic acid, directly or indirectly affect the regulation of growth and development of shoots of Ligustrum vulgare L. in response to stress factors, such as mechanical injury. The content of chlorogenic acid positively correlates with the increase in the length of internodes, which indicates its possible role in morphogenesis. It has been established that the profiles of phenolic substances allow identification of Ligustrum plants at the species level. Principal component analysis (PCA) and cluster analyses showed that the phenolic profiles of plants depend on their growing conditions and the urban environment. The study confirms the importance of plant trimming for stimulating plant growth. The significant adaptation potential of Ligustrum vulgare L. plants confirmed the suitability of their use in urban environments.