Variability of malondialdehyde content and yield elements in Triticum aestivum L. under heat stress conditions

Stefan Marković,Marija Petrović,Nevena Đukić

doi:10.5937/kgjsci2042045m

Abstract

Heat stress has a significant effect on the growth and development of cereals and affects cereals production by reducing the total yield. High temperatures in plants lead to oxidative stress, which creates reactive oxygen species that are specific to cause damage to the cell membrane. Due to the action of reactive oxygen species, lipid peroxidation can occur. For that reason, it is important to find cultivars that are more resistant to the harmful effects of heat stress and thus enable higher productivity. The aim of this study was to determine the effect of heat stress on the degree of lipid peroxidation and yield elements in leaves of 10 wheat cultivars, in order to select cultivars more resistant to heat stress. The degree of lipid peroxidation was determined spectrophotometrically by measuring the concentration of reactive substances of thiobarbiturate acid (mainly malondialdehyde - MDA). The cultivar Renesansa with the largest increase in MDA in conditions of high air temperature (2.848 µmol/g fw) was also characterized by the lowest yield (2870 kg/ha). On the other hand, the cultivar Talas with the smallest increase in MDA content (1800 µmol/g fw) in conditions of high air temperature showed significantly higher yield (3830 kg/ha). In this paper was shown that lipid peroxidation has a significant effect on yield reduction in the investigated wheat cultivars. Cultivars Talas, Apač, Hyfi, Mila and Salasar were characterized as cultivars more resistant to heat stress with higher yields. These results suggest that wheat cultivars were significantly different in their ability to respond to heat stress, which could be useful for the development of tolerant wheat cultivars in the breeding programs.

Highlights

Cereals are an important economic and food resource that’s the one of the most important nutrition in the daily human diet (FAROOQ et al, 2011)
Heat stress has a significant effect on the growth and development of cereals (PORTER and GAWITH, 1999) and affects wheat production, causing a reduction in yield of up to 71% (MUSTAFA et al, 2013)
The aim of this study was to determine the effect of heat stress on the degree of lipid peroxidation by measuring malondialdehyde content in the leaves of 10 wheat cultivars, to determine the yield of tested wheat cultivars and to compare them in order to select cultivars more resistant to heat stress

Summary

Introduction

Cereals are an important economic and food resource that’s the one of the most important nutrition in the daily human diet (FAROOQ et al, 2011). High temperatures in plants lead to oxidative stress creating reactive oxygen species (ROS) (SUZUKI and MITTLER, 2006). It is specific for reactive oxygen species to cause damage to the cell membrane (KUMAR et al, 2012), which can result in cell death and plant damage (LIU and HUANG, 2000; MARCUM, 1998). The increased concentration of formed ROS leads to lipid peroxidation causing damage to the cell membrane (BREUSEGEM et al, 2001; AMIRJANI, 2012). Stress resistance can differ significantly between wheat cultivars and different adaptation mechanisms can be activated among them

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