Variability of 27 Autosomal STR Loci for the Population of the Republic of Belarus Based on the Mass Parallel Sequencing Data

Abstract

Variability of 27 autosomal STR loci of the ForenSeq DNA Signature Prep Kit (Illumina) commercial panel was studied using the technology of mass parallel sequencing (MPS) in 733 unrelated individuals representing the population of the Republic of Belarus as well as a population base of MPS allele frequencies for expert probabilistic calculations in human identification and paternity establishment was evaluated. The agreement between genotypes obtained by MPS and capillary electrophoresis (CE) was 99.96%. The number of MPS alleles increased more than two times for eight loci (D12S391, D21S11, D2S1338, vWA, D3S1358, D8S1179, D13S317, D9S1122). Thirteen alleles detected were not included in the STRSeq BioProject catalog of the international online database STRbase 2.0. The random match probability of 27-locus MPS profiles decreased from 1.43 × 10–31 to 2.89 × 10–35, and the combined paternity index increased from 2.08 × 1010 to 3.25 × 1012 compared to CE data.