Abstract
An evaluation of diverse procedures for determining ash free dry weight (AFDW) has been carried out on a homogeneous periphyton biofilm. Results indicate a considerable degree of variability in AFDW depending on ignition time, ignition temperature and sample size. A standard method is called for; in the interim it is recommended that all three variables be reported to facilitate comparisons. Caution should be exercised when comparing results gained using dissimilar or incompletely quoted procedures.
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