Abstract
Grape canes are a waste product from viticulture that show potential as an industrially extractable source of stilbenes, which are valuable for medical and other purposes. In this work, grape canes collected in three consecutive years (2014–2016) at six different places in South Moravia, Czech Republic were extracted, and the contents of trans-resveratrol, trans-ε-viniferin, and r2-viniferin were determined by high-performance liquid chromatography. The study included three blue grape varieties of Vitis vinifera L. (Cabernet Moravia, Blaufränkisch, and Piwi variety Laurot) and four white grape varieties (Chardonnay, Green Veltliner, Piwi variety Hibernal, and Piwi variety Malverina). From the viewpoint of producing extracts with high stilbenes content, the Hibernal variety is clearly the best. The mean amounts of the stilbenes for this variety at all localities and for all three years were 4.99 g/kg for trans-resveratrol, 3.24 g/kg for trans-ε-viniferin, and 1.73 g/kg for r2-viniferin. The influence of vintage, locality, and variety on the amounts of stilbenes was studied using PCA analysis. In contrast to expectations, there was no strong impact of locality on stilbenes content. The differences were varietal for most varieties, regardless of the area of cultivation. Laurot and Hibernal varieties did differ significantly in that respect, however, as they exhibited clear dependence on location.
Highlights
In recent years, increasing attention has been focused on waste products generated in agriculture and in the food industry because such materials can be a source of valuable substances
One group of such substances is the stilbenes. These can be derived from grape canes, which are very rich in trans-resveratrol and trans-ε-viniferin
The varieties we focused on determining three dominant stilbenes in grape canes: trans-resveratrol, trans-εand six sampling sites are described in the Materials and Methods
Summary
In recent years, increasing attention has been focused on waste products generated in agriculture and in the food industry because such materials can be a source of valuable substances. It is difficult to compare the published data about the content of trans-resveratrol and trans-ε-viniferin in canes because various authors have studied different white and red varieties, including resistant and sensitive ones, and prepared samples of canes under different drying and storage conditions. Because all the aforementioned and future studies have required and will require a starting material such as trans-ε-viniferin in far greater amounts, a successful search for sources of these substances and a description of their distribution in different grapevine varieties with regards to sampling procedure and storage conditions is highly desirable and promising. To our knowledge, this is the first such comparing stilbenes contents of seven varieties of grape canes in six different places in South Moravia, comprehensive study of its kind. This is the first such comprehensive study of its kind
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