Variability in production of extracellular enzymes by different fungi isolated from rotten pear, peach and grape fruits

Shazia Parveen,Abdul Hamid Wani,Jahangir Abdullah Koka,Mohd Yaqub Bhat,Mohammad Afaan Fazili

doi:10.21472/bjbs.040804

Abstract

Fifteen fungal pathogens, viz. Penicillium chrysogenum, Penicillium expansum, Monilinia fructigena, Trichothecium roseum, Cladosporium herbarum, Aspergillus niger, Coprinus psychromorbidus, Alternaria alternaria, Phytophthora palmivora, Pythium sp., Fusarium solani, Alternaria sp., Monilinia sp., Guignardia bidwellii and Rhizopus stolonifer isolated from rotted fruits were evaluted for the production of different enzyme on solid culture media. All the isolates produced enzymes on the media used, 87.67% of the isolated fungi were found to produce amylases, 80.00% of the fungi produced lipases, 73.33% produced proteases, cellulases by 66.67% and pectinases by 60.00%. The array of enzymes produced differs between different fungi. Penicillium sp., Alternaria alternata, Rhizopus stolonifer and Alternaria sp. were found to produce all the tested enzymes on culture media. These pathogens are known to attack almost all the plants, fruits, vegetables and cause many diseases. The differences in the enzymatic production of different fungal pathogens represent their virulence and specificity in causing different fruit rot diseases.

Highlights

Microorganisms are invaluable sources of natural products for industrial and biomedical development (Turner and Aldridge, 1983)
Viz. Penicillium chrysogenum, Penicillium expansum, Monilinia fructigena, Trichothecium roseum, Cladosporium herbarum, Aspergillus niger, Coprinus psychromorbidus, Alternaria alternaria, Phytophthora palmivora, Pythium sp., Fusarium solani, Alternaria sp., Monilinia sp., Guignardia bidwellii and Rhizopus stolonifer were previously isolated from rotted pear, peach and grape fruits, stored on PDA medium at 4 °C in culture collection of Mycology and Plant Pathology Research Lab, Department of Botany, University of Kashmir and were screened for the production of different enzymes
It was revealed from the results that P. chrysogenum, A. alternata, R. stolonifer and Alternaria sp. were found to produce all the tested enzymes in culture media

Summary

Introduction

Microorganisms are invaluable sources of natural products for industrial and biomedical development (Turner and Aldridge, 1983) They are common sources of commercial enzymes due to their high production capability, low cost, susceptibility to genetic manupilation, high biotechnological interest, pharmaceutical products and medical therapy (Rao et al, 1998). Fungi in general are well characterized microorganisms due to their capacity to produce different extracellular compounds; some of them are biologically very important causing severe reactions in other organisms (Mustafa and Kaur, 2009). These compounds include different types of enzymes and biologically active molecules. The degredative enzymes produced by different fungi are important in food deterioration, breakdown of organic matter and host infection

Methods

Results

Conclusion