Variability in growth hormone stimulation tests

Abstract

The diagnosis of growth hormone deficiency (GHD) in adulthood has become increasingly important because of the approved indication for growth hormone (GH) substitution therapy in such patients. While GH stimulation tests are superior to single measurements of other growth factors or spontaneous GH secretion in the diagnosis in adults, the reproducibility and specificity of GH stimulatory tests are often described to be low. This is also the case with the insulin tolerance test. Many external factors, such as fasting, physical activity, heat exposure and sleep, are known to influence GH secretion. The stimulatory or inhibitory effect of these factors on GH secretion might, therefore, influence the GH provocative test and contribute to the variability in response. Age and body composition are also known to influence GH secretion, and these factors must be considered when evaluating GH test responses. However, age-related cut-off levels for GHD have not been defined. Obesity is still a complicating factor in the diagnosis of GHD, even though some GH tests have been able to distinguish between obesity and true GHD. Based on these complicating factors, the parameters of GH stimulatory tests are recommended to be defined and standardized to optimize reproducibility and specificity. Furthermore, such tests should be performed only in patients with firm evidence of pituitary disease.