Variability in accumulation of free proline on in vitro calli of four bean (Phaseolus vulgaris L.) varieties exposed to salinity and induced moisture stress

C醨Denas-Avila C醨Denas-Avila,Mr Morales-Vallarta,Jl Hern醤Dez-Pi馿Ro,Ma NEz-Gonz醠Ez,J Verde-Star,G Garc韆 D韆Z,R Foroughbakhch-P,S Mart韓Ez-Lozano,Ml Ml,H G醡Ez-Gonz醠Ez,Rk Maiti

doi:10.32604/phyton.2006.75.103

Abstract

This paper reports the genotypic variability in the accumulation of proline on the in vitro calli of bean cultivars exposed to induced water and salinity stress. Remarkable variations in the proline content were found among bean cultivars exposed to both stress factors.

Highlights

Bean (Phaseolus vulgaris L.) is an important high-protein food crop in Mexico and many Latin American countries of the world
Determination of free proline on in vitro calli exposed to salinity stress
This is in contrast to the report by Sawires et al (1997), cited by Maiti et al (2000) who reported that free proline in general increased with an increase in salinity

Summary

Introduction

Bean (Phaseolus vulgaris L.) is an important high-protein food crop in Mexico and many Latin American countries of the world. Techniques of in vitro tissue culture have been utilized as a valuable tool in the selection of crop cultivars for tolerance to salinity, and for studying the tolerance mechanisms to this stress factor (Revilla & Cañal, 1999; Kim & Song, 1984; Lupotto et al, 1988; Mongodi et al, 1988; Huang-Peiming & Ge-Koulin, 1989; Mahammed et al, 1992). Assays with this technique have demonstrated that increased salinity raised the accumulation of free proline in calli of bean. The objective of the present study was to study the effect of water and salinity stresses on the proline accumulation in cultivars of bean, and to establish its relation to the resistance mechanism of this species to these stress factors

Objectives

Methods

Results