Values of related biochemical indicators in the diagnosis of inflammatory bowel disease in children

Abstract

Objective To explore clinical values of related biochemical indicators in the diagnosis of inflammatory bowel disease (IBD) in children. Methods From January 1, 2015 to January 31, 2017, a total of 48 children with IBD were admitted into Chengdu Women′s & Children′s Central Hospital. They were divided into ulcerative colitis (UC) group (n=12, diagnosed with UC) and Crohn disease (CD) group (n=36, diagnosed with CD). Meanwhile, another 30 non-IBD children were enrolled into control group. The clinical data of all children were analyzed retrospectively, as well as levels of serum perinuclear anti-neutrophil cytoplasmic antibody (pANCA), anti-saccharomyces cerevisiae antibodies (ASCA) IgG, ASCA IgA, anti-mannobioside carbohydrate antibodies (AMCA) IgG, anti-chitobioside carbohydrate antibodies (ACCA) IgA, against bacterial flagellin cBir1 IgG antibodies (anti-cBir 1 IgG), and fecal calprotectin (FC). The age, body mass index (BMI), and levels of serum pANCA, ASCA IgG, ASCA IgA, AMCA IgG, ACCA IgA, anti-cBir 1 IgG and FC among 3 groups were compared by one-way ANOVA analysis, and SNK-q method was used to further comparison between different two groups. The constitution ratio of gender, and positive rates of serum pANCA, ASCA IgG, ASCA IgA, AMCA IgG, ACCA IgA, anti-cBir 1 IgG, and FC among 3 groups were compared by chi-square test. This study met the requirements of the World Medical Association Helsinki Declaration revised in 2013. Results ① No significant differences existed among 3 groups in term of age, BMI and constitution ratio of gender. ② There were significant differences among 3 groups in term of serum pANCA, ASCA IgG, ASCA IgA, AMCA IgG, ACCA IgA, anti-cBir 1 IgG, and FC (P<0.001). Further comparison between different two groups showed that, there were statistically significant differences in term of levels of serum pANCA, ASCA IgA, AMCA IgG, ACCA IgA, anti-cBir 1 IgG and FC (P<0.05). ③ There were significant difference among 3 groups in term of positvie rates of serum pANCA, ASCA IgG, ASCA IgA, AMCA IgG, ACCA IgA, anti-cBir 1 IgG, and FC (P<0.001). Conclusions Serum pANCA can be used as the first choice for the diagnosis of UC, and serum ASCA IgG, ASCA IgA, AMCA IgG, ACCA IgA, and anti-cBir 1 IgG can be used as specific indicators for the diagnosis of CD. However, whether these indicators can be used as specificity index for diagnosing and judging the prognosis of children with UC and CD still needs further study and confirmation. FC expression level is closely related to the progression and changes of children with IBD. Key words: Biological markers; Inflammatory bowel disease; Antibodies, antineutrophil cytoplasmic; Diagnosis; Serologic tests; Child