Value of runt related transcription factor 3 gene CpG island methylation in induced sputum in condition and prognosis evaluation for lung adenocarcinoma

Abstract

Objective To study the value of runt related transcription factor 3 (Runx3) gene CpG island methylation in induced sputum in condition and prognosis evaluation for lung adenocarcinoma. Methods Methylation specific polymerease chain reaction (MSP) was used to detect the Runx3 gene promoter methylation by induced sputum in patient with lung adenocarcinoma (LAC group) and non-cancer patients (CON group). The Runx3 gene CpG island methylation rate in different clinicopathological factors was compared. The R0 radical resection, disease-free survival time and 3-year survival rate between patient with Runx3 gene CpG island methylation and unmethylation were compared. Results The Runx3 gene promoter methylation rate in LAC group was significantly higher than in CON group (62.5% vs. 2.5%, P<0.05). The Runx3 gene promoter methylation rate in pleural effusion, degree of differentiation, tumor diameter, lymph node metastasis, distant metastasis and cTNM staging were significantly different (χ2=5.861, 4.417, 5.273, 4.110, 10.240, 10.559, all P<0.05). The R0 radical resection (72.0% vs. 93.3%), disease-free survival time [(1.7±0.4) years vs. (2.4±0.6) years] and 3-year survival rate (40.0% vs. 66.7%) in patient with Runx3 gene CpG island methylation were significantly lower than in patients with Runx3 gene CpG island unmethylation (χ2=4.083, t=6.261, χ2=4.320, all P<0.05). Conclusion Runx3 gene CpG island methylation in induced sputum correlates with lung adenocarcinoma’s condition and prognosis. Key words: Lung adenocarcinoma; Runt related transcription factor 3; Methylation