Value of combined detection of SSA antibody, anti-SSB antibody, and anti-α-fodrin antibody in diagnosis of Sjögren’s syndrome

Abstract

Objective To determine the IgA and IgG expressions of combined detection of SSA antibody, anti-SSB antibody, and anti-α-fodrin antibody in Sjogren’s syndrome, and to investigate their clinical value in the diagnosis of Sjogren’s syndrome. Methods Patients and healthy subjects, who were hospitalized in our hospital between October 2012 and August 2015, were included in the study. The anti-α-Fodrin IgA and IgG antibodies were determined by enzyme-linked immunosorbent assay (ELISA) . Anti-SSA antibody and anti-SSB antibody were detected by immunoblotting. There were a total of 108 cases of primary Sjogren's syndrome (pSS) , 61 secondary Sjogren’s syndrome (sSS) and 98 normal healthy subjects in the control group. Results (1) The SS sensitivity diagnosed by the combined detection of anti-α-lytic antibody IgA, anti-SSA antibody and anti-SSB antibody was higher than that of the separate detection (P<0.05) , especially the diagnosis of pSS. (2) The positive rates of serum anti-α-Fodrin IgA and IgG antibodies in the Sjogren’s syndrome were 78.0% and 40.8%, respectively, which were significantly higher than those of SLE and RA, and there were statistically significant differences (P<0.05) . (3) The serum anti-α-lytic protein antibody was more than 20% positive in the serum of Sjogren’s syndrome patients with negative serum anti-SSA antibody and anti-SSB antibody. (4) The disease-related indicators in patients with positive anti-α-Fodrin IgA and IgG antibodies were higher than those in the negative patients. Conclusion Anti-α-Fodrin antibody has certain significance in the diagnosis of Sjogren’s syndrome, and its type detection is more favorable for the early diagnosis of Sjogren’s syndrome. Anti-α-fodrin IgA antibody in pSS patients is more common than the IgG antibody. The combined detection of anti-α-fodrin antibody, anti-SSA antibody and anti-SSB antibody can improve the sensitivity of Sjogren’s syndrome diagnosis, providing certain for t diagnostic value for conventional antibody-negative patients. Key words: Sjogren’s syndrome; anti-α-fodrin antibody; anti SSA antibody; anti SSB antibody; enzyme-linked immunosorbent assay (ELISA)