Value addition ofSamia ricini (Eri) pupae derived proteins: production and characterisation of antioxidant, ACE and DPP-IV inhibitory hydrolysates

Abstract

This study aims to validate the production of multifunctional peptides from indigenously available edible nonmulberry silkworm (Samia ricini) pupae under the effect of specific enzymatic hydrolysis settings, simulated gastric and intestinal fluids. Eri pupae proteins comprising 40.81% constituents with molecular weight (MW)>10 kDa and 48.68% within 10-3 kDa are most preferable substrates for alcalase and thermolysin among other enzymes; based on the effect of relationships between 0.5-3.0% enzyme/substrate ratios and 0-24 h of hydrolysis period on rate of hydrolysis, reduced Km value (6.07 mg/ml) of alcalase and higher kcat/Km value (0.42 ml/mg/min) demonstrated by thermolysin. hydrolysates demonstrating the best angiotensin converting enzyme (ACE) inhibition (alcalase hydrolysate, 3.0%, 3 h (H1′), papain hydrolysate, 3.0%, 5 h (H2′), pepsin hydrolysate, 3.0%, 5 h (H3′)), DPPH scavenging potential (H1′ and H3′), ABTS scavenging capacity (H2′, H3′ and thermolysin hydrolysate, 3.0%, 5 h (H4′)) and DPPIV inhibition (H4′) were selected and compared their stability and peptides behaviour under gastrointestinal environment with non-hydrolysates. This study has provided enough evidence that there exists an opportunity to utilise H3′ and H4′ for practical application due to its maintenance of ACE and DPP-IV inhibition potential after digestion compared to whole Eri pupae flour or its concentrate. Thus, this preliminary scientific segment of allegation suggested that the possibility of exploration of sericulture industry derivatives through enzymatic processing not only helps in waste procurement but will also provide the opportunity to innovate cost-effective value-added products in the form of bioactive protein hydrolysates.