Évaluation of the anti-malarial properties of the combined aqueous and alcoholic extract of Heliotropium indicum and Parquentina nigrescencs

Abstract

This research investigates the potential antimalarial efficacy of combined extracts derived from Heliotropium indicum and Parquetina nigrescens, considering their traditional applications in addressing various ailments, including malaria. The primary objective is to assess the suppressive antimalarial activity of the combined extracts and compare it with individual extracts, utilizing toxicity assessments and syrup formulations. The study includes LD50 assessments to confirm the safety of the extracts, employing doses beyond 5000mg/kg. Suppressive assays are conducted on mice, evaluating both individual and combined extracts, with varying doses. White mice from a reputable source are used for the experiments. The extracts are prepared as syrups, and different doses are administered to the mice for toxicity and antimalarial assessment. Parasitemia is evaluated through blood films, and statistical analysis is performed. The obtained data are analyzed using one-way ANOVA and Student-Newman Keul’s test to determine statistical significance. Results are expressed as mean ± SEM. LD50 assessments reveal the safety of the extracts, and suppressive assays demonstrate significant antimalarial effects for both individual extracts at distinct doses. The combined aqueous extract shows promising outcomes, indicating potential synergistic interactions. Traditional plant-based remedies, such as Heliotropium indicum and Parquetina nigrescens, hold promise for developing innovative antimalarial strategies. The combined extract exhibits potential as a complementary antimalarial intervention, warranting further exploration and research in the field of malaria management.