Abstract
Polyphenols are bioactive molecules with much scientific attention because of their multiple biological activities. This study aims to compare the phenolic content in the extracts hydro-methanolic needles and roots bark of Juniperus oxycedrus subsp. oxycedrus and their antioxidant activity by five different methods (total antioxidant capacity, DPPH, ABTS, β-carotene and ferric reducing power). Results showed that needles extract exhibited the highest polyphenol (133.08 ± 4.1 GAE.g−1DW) flavonoid (61.5 2 ± 3.1 CEf·g−1DW) and tannin (26.43 ± 2.6 CEt.g−1DW) contents. Both of extracts revealed a significant antioxidant activity. However, the roots bark extract displayed the highest capacity with the lowest IC50 value (2.9 ± 0.2 μgmL−1) in DPPH scavenging method. Overall, this paper established that the roots bark possess high in vitro antioxidant potency although they possess less phenolic compounds than needles. Therefore, our results can confirm that the high content of total phenols in the extracts does not explain the strong antioxidant properties. The nature of these compounds and synergistic interactions can influence this ability.
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