Évaluation d'une nouvelle technique de dosage des immunoglobulines spécifiques E des protéines du lait de vache

Abstract

Topics Diagnosis of cow's milk intolerance (CMI) is founded upon assay of specific IgE against cow's milk proteins whose ImmunoCap technic from pharmacia diagnostics (Phadia) allows to quantify from 0.1 KUA/l. We retrospectively studied the advantages of this extent of the scale of concentrations with regard to that traditionally scale restricted at 0.35 kUA/l and we verified the forecasted threshold of 0.1 kUA/l. Material and methods We selected 68 serums from 63 children, 32 with CMI confirmed, 12 with probable CMI and 19 controls. Anti alpha lactalbumin, beta lactoglobulin and casein IgE had been measured for every sample on UniCap 100, with a couple of dosage protocols: the usual one which allowed to quantify IgE from 0.35 to 100 kUA/l and the new one with a scale of calibration fit for quantify from 0.1 kUA/l. Results The threshold of this new protocol had been determined on 0.1 kUA/l for the three allergens. The two protocols were well correlated. For 21 among 68 samples (31%), the new calibration permitted to calculate concentrations with specific IgE but it was obtained < 0.35 KUA/l with the old one. These samples correspond with 10 confirmed CMI, 3 probable CMI, 7 controls, among which 5 isolated gastro oesophageal reflux GER. Conclusion Results of this study showed us that the new IgE dosage protocol can be a rearlier diagnosis tool of a cow's milk sensitization. Prospective study is needed to confirm. We asked about possible link between sensitization and GER.