Valorization of spent mushroom substrate in combination with agro-residues to improve the nutrient and phytohormone contents of vermicompost

Abstract

In recent years, enormous amounts of spent mushroom substrate (SMS) have been generated because of the rapid development of mushroom production. Since the conventional disposal methods of these residues can cause serious environmental problems, alternative waste management techniques are required to ensure sustainable agriculture. However, SMS might be not suitable for vermicomposting when used alone. Therefore, the primary purpose of this study was to investigate the effect of Azolla microphylla (Azolla) biomass, eggshells, fruit peels, and cassava pulp on the biodegradation process of SMS. The results showed the treatments supplemented with cassava pulp and fruit peel waste improved the growth of earthworms, while the carbon-to-nitrogen ratio of these vermicomposts decreased significantly (p < 0.05) due to the improved total nitrogen contents (7.64 g kg−1 and 6.71 g kg−1). Concerning the degradation process and the vermicompost quality, the addition of these agro-residues facilitated the enzyme activities (cellulase, urease, and alkaline phosphatase) and increased the total macronutrient (P, K, Mg, and Ca) and phytohormone (fruit peel waste: AA, GA3, and cytokinin; cassava pulp: cytokinin) contents of the final products compared to the control treatment. On the other hand, Azolla had no additional effect on the fecundity and growth of Eudrilus eugenia. Meanwhile, the treatment supplemented with eggshells was high in Mg (7.15 g kg−1) and Ca (305.6 g kg−1). Overall, the combined decomposition of SMS-based bedding material with Azolla, eggshells, fruit peel waste, and cassava pulp resulted in mature organic fertilizers with improved chemical properties.