Valorization of Food Waste Slurry as Potential Candidate for Lipid Accumulation: A Concept of Oleaginous Bio-Refinery

Abstract

In the current state of huge waste production and energy crisis, there is a need to find additional alternate energy resources and options for waste management. The present study was designed to measure the potential of different fruit wastes to serve as substrate for lipid accumulation in oleaginous bacteria. For this purpose, three novel bacterial strains (AF3, KM1 and KM10) isolated from the crude oil samples were systematically compared for their lipid accumulation potential using three types of waste including orange waste (OW), mango waste (MW) and apple waste (AW). Using waste as sole substrate, it was observed that maximum lipid accumulation by each strain was above 20%, which confirms that the bacteria belong to the oleaginous group. However, each bacterial isolate represented differential accumulative capacity with varying organic matter removal efficiency. Maximum lipid accumulation was achieved by KM10 (>25%) with AW as substrate, and KM1 (>24%) with MW as substrate; however, AF3 represented only 21% lipid accumulation using AW as substrate. Similarly, the maximum removal efficiency was recorded for KM10 in AW, followed by OW, where >60% and >50% of volatile solids (VS) removal, respectively, were achieved over the period of 7 days of incubation. This showed that the oleaginous strains also exhibit excellent waste treatment efficiency. The 16s RNA gene sequencing results showed that these KM1 and KM10 strains were Serratia surfactantfaciens and Serratia liquefaciens. In the end, a circular economy model was presented to highlight the significance of the mechanisms, which offers dual benefits over the linear economy model. Overall, the findings of the present study revealed that the novel oleaginous strains not only provide considerable lipid accumulation, but are simultaneously capable of low-cost waste treatment.