Abstract
Abstract Aim Evaluation of the medium components and conditions for the optimization of xylanase production in submerged fermentation by Trichoderma koeningi isolate (KT006533). Methods Trichoderma koeningi was identified by the 18s rRNA. NaOH pre-treated corn cobs were used in order to reduce the crystallinity of corn cobs. De-oiled cakes proximate composition was analyzed according to AOAC. Plackett-Burman design (PBD) was used to screen the 19 media variables that affect xylanase production and optimized the media by central composite design (CCD). Results The isolate was identified as Trichoderma koeningi. Among the 19 variables more effective variables on xylanase production was mustard cake, xylose, FeSO4·7H2O, ZnSO4·7H2O and pH. The optimization of xylanase and protein were quantified as 74.84 U/mL and 2.94 mg/mL, respectively, owing to the influence of the above components at g/L 3.5, 6.0, 0.06, 0.04 at levels respectively and pH 6.5, by using a CCD. The coefficient of determination (R2) is 0.92%. The correlations between the actual and coded results are found to be good. Conclusion NaOH pre-treated corn cobs were used with mustard cake powder as fermentation medium constituent to induce the xylanase production. From the results we conclude that mustard cake induced the xylanase production.
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