Valorisation of bio-derived fluorescent carbon dots for metal sensing, DNA binding and bioimaging

Abstract

Carbon dots are quasi-spherical and zero dimensional nanomaterials with unique optical and electronic properties. In this work, a facile and sustainable strategy was employed to synthesise nitrogen doped carbon dots from Terminalia chebula via hydrothermal treatment with a quantum yield of 19.9%. The structural and optical properties of nitrogen doped carbon dots (N-CDs) were studied by UV–Visible absorption and fluorescence spectroscopy. The surface functional groups, average particle size and elemental analysis were assessed with the help of Fourier Transform Infra Red spectroscopy, High Resolution Transmission Electron Microscopy and Energy Dispersive X-ray analysis respectively. The N-CDs exhibited excitation dependent emission upon irradiation with UV light, pH stability over neutral range and excellent photostability. The average particle size of the synthesised N-CDs was found to be 3.56 nm. The fluorescence intensity of the N-CDs quenched linearly with increase in concentration of Fe3+ ions. The limit of detection (LOD) of N-CDs with Fe3+ ions was calculated to be 4.5 nM using Stern-Volmer plot. The fluorescence was restored by addition of EDTA to Fe3+ coordinated N-CD system. Further, the synthesised N-CDs interacted with ct-DNA through intercalative mode and the binding constant calculated using the Benesi Hildebrand plot was 1.78 × 108 mg/mL. The cytotoxicity of N-CDs was evaluated using MTT assay. The excellent biocompatible and less toxic nature of N-CDs was extrapolated to serve as fluorescent probes for imaging E.coli and SKMEL cells. From the results of this work, it is evident that the synthesised N-CDs can be used to develop efficient fluorescent metal sensors. The fluorescent property of N-CDs enables it to find extension as a potential curative drug, an efficient patterning agent and an effective biomarker to image biological cells causing no damage to normal cells.