Abstract
Using LC/MS, isoflavones (glycitin, genistin, tectoridin, glycitein, genistein, and tectorigenin) known as Puerariae Flos marker compounds were identified. The simultaneous analysis and validation of six marker compounds were performed using the HPLC-DAD method. To validate the analysis method of six marker compounds 1–6, the linearity was showed in the calibration curve as coefficient of correlation (R2) range of 0.9997–1.000. The limit of detection (LOD) and limit of quantitation (LOQ) of six marker compounds were measured in the ranges of 0.2720–0.7884 µg/mL and 0.8243–2.3890 µg/mL, respectively. Intermediate precision, repeatability, and accuracy evaluations according to the Ministry of Food and Drug Safety (MFDS) guidelines showed that the relative standard deviation (RSD) less than 1% and the stability less than 2%, respectively. Also, the simultaneous validation method is attributed to analysis of the different isoflavones Flos ne compounds in Puerariae extract. It could be potentially used as sources of pharmaceutical and natural antioxidant ingredients.
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