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Abstract
A new liquid chromatographic method with an ion trap detector for the determination of chloramphenicol residues in animal feed is described. The developed method consists of an organic extraction and a two-step cleanup. For the cleanup, solid phase and liquid–liquid extractions were applied to minimize the ion suppression effects. One precursor and three product ions were monitored obtaining 5.5 identification points, which provided the method with a very high selectivity/specificity and gave a great confidence to the results. The described method was fully validated according to the requirements of the 2002/657/EC European Decision and the estimated decision limit and detection capability were 6 and 8 μg Kg−1, respectively.
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