Validation of the analytical method for determination of tetracycline residues in poultry chest, thigh and liver by HPLC-DAD technique

Abstract

An easy and quite quick method was developed to determine tetracycline residues in poultry tissues with a good separation and a high sensitivity. This method permitted to analyze various tissues (thigh, chest, liver). Validation of the analytical method is investigated to check if the method’s analytical objective is accomplished, which is obtaining analytical results with an appropriate degree of uncertainty or a good level of confidence. The validation measures of the analytical method were recorded as follows: Limit of Detection (LOD) 0.451 ppb, Limit of Quantification (LOQ) 1.502 ppb, linear correlation coefficient 0.999066 within a range of concentrations between (100.0-300.0 ppb), Decision Limit CCα 201.946, 202.763, 603.231 ppb, Detection Capability CCβ 203.892, 205.527, 606.462 ppb for Poultry chest, thigh and liver, respectively, recovery percentages of Tetracycline at a concentration of 200.0 ppb for 20 sample Rec.% (88.966 - 91.055%), (84.623 - 87.667%), (82.198 - 83.688%) for Poultry chest, thigh and liver respectively with a percentage relative standard deviations (RSD%) of < 1 %.