Abstract
The covid-19 pandemic has accelerated the use of direct-to-consumer offerings of at-home, mail-in kits for sperm DNA fragmentation. However, mail-in semen collection kits involve incubation in transport media and overnight shipping. DNA fragmentation can be confounded by multiple extrinsic factors such as storage temperatures, transportation media, handling conditions, time after ejaculation, and oxidative stress. The objective of this study was to validate the sperm chromatin dispersion test using at-home, mail-in sperm collection kits. To do so, we evaluated and assessed the effect of transportation media and shipping on sperm DNA integrity using a Halosperm® G2 kit in normozoospermic human sperm samples.
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