Abstract
BackgroundThe role of n-3 polyunsaturated fatty acids (PUFAs) in breast cancer is not clear and under debate. To explore this relationship it is important to have proper validated dietary assessment methods for measuring the intake of n-3 PUFAs. The aim of the current study is to validate two different methods used to assess the intake of selected n-3 PUFAs as well as food sources of long-chained n-3 PUFAs. Also, we aim to study how stable the intake of fatty acids is during breast cancer treatment.MethodsThe study-population was patients with breast cancer (Stages I-II) or ductal carcinoma in situ (DCIS-grade III) undergoing treatment (n = 49) in Norway. Dietary intake was assessed by two self-administered methods, a 256 food item food frequency questionnaire (FFQ) and a 7-day pre-coded food diary (PFD). The FFQ was administered presurgery and twelve months postsurgery, and the PFD was administered shortly after surgery (10 +/− 2 days), six and twelve months postsurgery. Fasting blood samples (presurgery, six and twelve months postsurgery) were analysed for serum phospholipid fatty acids, a biomarker for intake of n-3 PUFAs.ResultsMean (SD) age was 54.2 (7.8) years at diagnosis, and the mean (SD) body mass index (BMI) was 24.8 (3.4) kg/m2. Correlation coefficients between dietary intakes of n-3 PUFAs measured with the FFQ and the PFD ranged from 0.35 to 0.66. The correlation coefficients between the PFD and the biomarker (serum phospholipid n-3 PUFAs) as well as between the FFQ and the biomarker demonstrated stronger correlations twelve months after surgery (ρ 0.40–0.56 and 0.36–0.53, respectively) compared to around surgery (ρ 0.08–0.20 and 0.28–0.38, respectively). The same pattern was observed for intake of fatty fish. The intake of n-3 PUFAs did not change during treatment assessed by the FFQ, PFD or biomarker.ConclusionThese results indicate that the FFQ and the PFD can be used to assess dietary intake of fish and n-3 PUFAs in breast cancer patients during breast cancer treatment. Still, the PFD shortly after surgery should be used with caution. The diet of patients undergoing breast cancer treatment was quite stable, and the intake of n-3 PUFAs did not change.
Highlights
The role of n-3 polyunsaturated fatty acids (PUFAs) in breast cancer is not clear and under debate
The full fatty acid composition is presented in the Additional file 1: Table S1
Intake of most nutrients, including n-3 PUFAs, did not change during treatment assessed by the food frequency questionnaire (FFQ) or pre-coded food diary (PFD)
Summary
The role of n-3 polyunsaturated fatty acids (PUFAs) in breast cancer is not clear and under debate. The role of n-3 polyunsaturated fatty acids (PUFAs) in the diet of women diagnosed with breast cancer is not clear [3,4,5]. To be able to investigate the n-3 PUFAs role in breast cancer prognosis or study dietary changes after diagnosis it is important to have proper methods to measure the dietary intake. The food frequency questionnaire (FFQ) on the other hand attempts to estimate usual dietary intake in one administration, is usually a lower burden on the respondent and the investigator and the cost of administration and handling is relatively low [13]. The FFQ is a closed method and may give fewer details on specific food types and cooking methods, and is more prone to measurement errors including recall errors as it is memory-dependent [13]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
