Abstract
The tropical shads found in Brunei Darussalam was identified using a morphological analysis, and the identification was further validated by an analysis of the shads’ 16S ribosomal RNA gene sequences. Because of the difficulty of accurately identifying tropical shads solely on morphological analyses, previous studies had reported the occurrence of the tropical shads Tenualosa toli (Valenciennes, 1847) and T. macrura (Bleeker, 1852) in Malaysian waters of the Borneo Island. However, based on the morphological key characters and geographical distribution, all specimens were identified as T. reevesii (Richardson, 1846). Thereafter, all specimens were identified as T. toli, which was confirmed by the molecular genetic analysis. This study found the occurrence of T. toli in Brunei Darussalam. The present study also suggests that accurate tropical shad species identification is needed to validate by molecular genetic analysis after morphological observation.
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