Validation of Microcirculatory Parameters Derived from the Standard Two-Compartment Model with Murine Xenografts Model

Septian Hartono,Richard Weijie Ong,Laurent Martarello,Sidney Wing Kwong Yu,The Hung Huynh,Tong San Koh,Choon Hua Thng,Quan Sing Ng,Tony Kiat Hon Lim

doi:10.1155/2015/769849

Abstract

The purpose of this study was to validate DCE-MRI parameters such as blood flow (F), permeability surface area product (PS), fractional intravascular space (v1), and fractional extracellular extravascular space (v2), obtained using a standard two-compartment model against other established analysis methods and histological indices. DCE-MRI datasets of 28 mice implanted with various human cancer xenografts were acquired and analyzed. Statistically significant correlations were found between the parameters derived from the standard two-compartment model (v1, v2, F, and PS) with the histological markers of intravascular and interstitial space and with the corresponding flow and permeability estimates obtained by the initial slope method and Patlak plot, respectively.

Highlights

It is important to validate the parameters derived from DCE-MRI against established techniques and to ascertain that the DCE-MRI parameters reflect the actual microcirculatory state and pathophysiology of the tumors imaged
With histological according indices of the tumor microvasculature. to Generalized Kinetic (GK) model theory, Ktrans incorporates both the effects of blood flow and vessel permeability [5] and a recent simulation study [6] has shown that Ktrans has a significant positive correlation with blood flow, permeability, and blood volume, as well as a significant negative correlation with interstitial volume
More complex tracer kinetic models such as the distributed parameter models [10] are capable of separately estimating blood flow and permeability

Summary

Introduction

It is important to validate the parameters derived from DCE-MRI against established techniques and to ascertain that the DCE-MRI parameters reflect the actual microcirculatory state and pathophysiology of the tumors imaged. The standard two-compartment model [7,8,9,10] describes tissue microcirculation with distinct parameters, namely, blood flow (F), vessel permeability surface area product (PS), fractional vascular volume (V1), and fractional interstitial volume (V2). These physiological parameters can be readily validated by comparison with appropriate histological markers or other established tracer techniques.

Objectives

Methods

Results

Conclusion