VALIDATION OF HPLC AND UV VISIBLE METHODS FOR FEW SELECTED BLOOD PRESSURE LOWERING DRUGS AND THEIR FORMULATIONS

Abstract

Objective: A simple, precise and accurate RP-HPLC method has been developed and subsequently validated for simultaneous estimation of Aliskiren Hemifumarate and Nicardipine Besylate from their combination dosage form. Aliskiren and Nicardipine are widely used antihypertensive drugs at present but their analytical methods are very costly and very complex to simplify the methods with increasing sensitivity new methods were developed which are simple, precise, eco-friendly, less time consuming, rapid and fast and economically chief. Methods: First standard curve was plotted then the method is validated by using recovery studies, linearity, correctness and reproducibility, robustness, ruggedness, detection limit, quantification limits, stability studies etc. The validated technique has been with success used for stress testing analysis of Aliskiren and Nicardipine. Results: The stress testing studies revealed that the tactic was with success utilized to resolve the degraded product from the sample. From the peak purity profile it had been demonstrated that there was no interference of degradation product and the purity of angle were found to be but the purity of threshold. This work was undertaken with an aim of developing HPLC and Specrophotometric techniques for analysis of Aliskiren and Nicardipine. Number of trials was taken for selection of column and M. Phase’. The proposed method was validated as per the ICH and USP guidelines. Conclusion: The stress testing studies revealed that the tactic was with success utilized to resolve the degraded product from the sample. From the peak purity profile it had been demonstrated that there was no interference of degradation product and the purity of angle were found to be but the purity of threshold. Peer Review History: Received 11 February 2017; Revised 13 March; Accepted 14 March, Available online 15 March 2017 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file: Reviewer's Comments: Average Peer review marks at initial stage: 5.5/10 Average Peer review marks at publication stage: 7.5/10 Reviewer(s) detail: Dr. Mohamed Salama, Modern University for Technology & Information, Egypt, salama47@yahoo.com Dr. Mohammad Shaheen Khan, University Malaysia Sabah, Malaysia, shaheenchem@gmail.comSimilar Articles: ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF OMEPRAZOLE AND ASPIRIN USING REVERSE PHASE HPLC METHOD IN BULK AND DOSAGE FORM