Validation of Gender Specific CAPS Marker in Turkish Fig (Ficus carica L.) Collection and F1 Progenies

Hatice IKTEN,Yeliz YILMAZ

doi:10.15835/nbha47311499

Hatice IKTEN, Yeliz YILMAZ

Open Access

https://doi.org/10.15835/nbha47311499

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Abstract

In most dioecious plants, distinguishing male and female progenies is not possible until flowering or fruiting stage. The fig (Ficus carica L.) is such a plant where distinguishing male and female plants at the seedling stage can accelerate fig-breeding programs. An orthologue of RAN1 loci was reported to be associated with sex determination in fig (Mori et al., 2017). The objective of this study is to validate this locus on Turkish fig germplasm collection and F1 population obtained from a cross between female genotypes ‘Bursa Siyahi’ and male genotype ‘Ak Ilek’. A total of 144 genotypes from germplasm collection and 115 F1 individuals were tested with CAPS (cleaved amplified polymorphic sequences) marker following the Mori et al. (2017). The loci produced a 315bp amplification product from all genotypes. PciI digestion of PCR products resulted in 100% concordance between phenotypes and molecular tests. On the other hand, HpyCH4IV enzyme digestion of 8 female genotypes resulted in false negatives among the tested materials. Therefore, despite overall results show that the locus is suitable for gender selection of plants at the seedling stage in the breeding programs, care should be taken when HpyCH4IV enzyme is to be employed for CAPS assay.  ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

Highlights

The fig (Ficus carica L.) is an economically important crop in the world especially in countries bordering the Mediterranean Sea, Red Sea, Arabian Sea and U.S.A (Storey, 1975)
The aim of the present study was to validate previously developed sex-linked CAPS markers by Mori et al (2017) on the male and female plants obtained from fig genetic resources collection of Turkish origin and further test MAS applicability of locus on F1 population
An orthologue of RAN1 locus was reported to be associated with sex determination in fig (Mori et al, 2017)

Summary

Introduction

The fig (Ficus carica L.) is an economically important crop in the world especially in countries bordering the Mediterranean Sea, Red Sea, Arabian Sea and U.S.A (Storey, 1975). As in most dioecious plants, only the female fig plants are commercially valued for production and it is not possible to distinguish the male and female trees until they pass through the juvenile period and produce fruits. Distinguishing male and female plants at the seedling stage could accelerate breeding studies by reducing the labor, time and other expenses in breeding programs (Storey, 1975). Molecular markers are easy to practice, more reliable, not effected by environmental conditions and plant growth stage (Francia et al, 2005) and marker assisted selection has become an important tool in selection of progenies carrying the desired characters (Sestras et al, 2009)

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