Abstract
The accreditation of a laboratory requires the description of methods used in standard operating procedures and their validation. In the present work, an enzyme‐linked immunosorbent assay (ELISA) in a double‐antibody method for the detection of potato virus antigens in sap was subject to validation. Commercial virus‐specific antibodies were used for the coating and the virus was detected with commercial enzyme‐labelled antibodies. Sap samples derived from plants naturally infected with potato virus M (PVM), potato virus Y (PVY), potato virus S (PVS) or potato leafroll virus (PLRV) were examined in order to evaluate repeatability, analytical sensitivity and analytical specificity of the ELISA method in an experimental series (EPPO Standard PM 7/84). No tendency to carry‐over in a multi‐channel pipette system was observed in consecutive readings of microtitre plates.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
