Validation of antibody-based assays for regulatory peptides: Do it once, get it right, and exploit the under-appreciated benefit of long-term antibody stability

Abstract

Many submitted manuscripts utilizing antibody-based assays for biologically active peptides frequently neither include nor cite adequate validation data with the risk that the report is adding to the reproducibility crisis in biological research. On the basis of recent experience in re-characterizing in a radioimmunoassay format a polycolonal antibody to gastrin that was first raised nearly five decades ago, it is argued that some antibodies can be stable for very many decades. Researchers concerned about the reproducibility of data using antibodies in assays for regulatory peptides should therefore note that by rigorous validation at an early stage they may not only contribute to the resolution of the reproducibility crisis but also establish a resource that could be useful for very many years.