Validation of analytical methods for organochlorine pesticide detection in shellfish and cephalopods by GC-MS/MS.

Abstract

This study validated the analysis of organochlorine pesticides (OCPs) in shellfish and cephalopods using a gas chromatograph equipped with a mass spectrometry (GC-MS/MS), and monitored residual pesticide levels. The QuEChERS method was used to analyze OCPs and was validated by checking the linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, and precision. Octopus minor and Venerupis philippinarum, were purchased from four cities in the South Korean peninsula. The LOD values were 0.10-0.80ng/g in shellfish and 0.21-0.77ng/g in cephalopods, while the LOQ values were 0.31-2.41ng/g in shellfish and 0.63-2.33ng/g in cephalopods. Accuracy ranged from 83.5 to 117.4% and 79.8 to 118.4%, and precision ranged from 0.3 to 27.5% and 1.2 to 27.9%, in shellfish and cephalopods, respectively, conforming to the Codex Alimentarius Commission guidelines. Although residual OCP levels were below detection limits, the QuEChERS method may be effective for analyzing the OCPs in shellfish and cephalopods.