Abstract
A liquid chromatography (LC) method was developed and validated for identification and quantification of polyphenols in aqueous extractive solution from Ilex paraguariensis (erva‐mate), in agreement with the ICH requirements for analytical methods. The analysis was performed using a RP 18 column, in gradient solvent. Chlorogenic acid (CLOA) and rutin (RU) were used as external standards. The standard curves for CLOA and RU were linear with correlation coefficients higher than 0.9980. The LC method showed excellent performance in separating seven peaks. The method showed excellent repeatability (R.S.D.<2.0%) and accuracy (CLOA=97.4 and RU=104.0%). Besides CLOA and RU, six other constituents were detected, which were identified by LC‐MS/MS based on their mass spectra in full scan mode and retention times compared with those available in the literature data. The analytical method was successfully applied for quantifying polyphenols in four different extractive solutions from erva‐mate, a decoction (ES), an infusion (ESI), and an extractive solution obtained by turbo extraction with water (TE1) or ethanol 40% (v/v) (TE2). The last one presented the highest polyphenol concentration.
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