Abstract
ABSTRACT A high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method was validated for the study of bioactive amines in chicken meat. A gradient elution system with an ultraviolet detector was used after extraction with trichloroacetic acid and pre-column derivatization with dansyl chloride. Putrescine, cadaverine, histamine, tyramine, spermidine, and spermine standards were used for the evaluation of the following performance parameters: selectivity, linearity, precision, recovery, limits of detection, limits of quantification and ruggedness. The results indicated excellent selectivity, separation of all amines, a coefficient of determination greater than 0.99 and recovery from 92.25 to 102.25% at the concentration of 47.2mg.kg-1, with a limit of detection at 0.3mg.kg-1 and a limit of quantification at 0.9mg.kg-1 for all amines, with the exception of histamine, which exhibited the limit of quantification, of 1mg.kg-1. In conclusion, the performance parameters demonstrated adequacy of the method for the detection and quantification of bioactive amines in chicken meat.
Highlights
Chicken meat has high nutritional value and is rich in proteins, vitamins and minerals
The consumption of food with high amine content can be harmful to health, potentially leading to histamine poisoning, migraine, or hypertensive crisis (Palencia et al, 2011)
The study of biogenic amines is important due to their toxicity and because they can be used as food quality indicators
Summary
Chicken meat has high nutritional value and is rich in proteins, vitamins and minerals. This product is highly perishable and is. The study of biogenic amines is important due to their toxicity and because they can be used as food quality indicators. In this sense, the development of methodologies able to detect toxic compounds has been increasingly pursued to ensure that food products are not associated with any type of risk for the consumer
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
