Validation of an efficient analytical method for quantification of tetracycline antibiotic residues in egg samples along with investigating their decontamination using ultraviolet waves

Abstract

ABSTRACT This study describes the development and application of a dispersive solid-phase extraction procedure combined with dispersive liquid – liquid microextraction for the extraction of tetracycline antibiotics from egg samples. In this method, a bio-metal organic framework was prepared and used in extraction of the analytes from sample solution. In this method, the sorbent synthesis was relatively easy and its crosslinker was vitamin B3 which is a green chemical compound. The sorbent provides particles can extract the analytes via interactions such as surface adsorption, occlusion van der waals and π–π interactions. The extraction procedure was done by mixing trichloroacetic acid with the egg sample to remove the sample proteins and then the synthesised sorbent at mg – level was added to the supernatant phase and the mixture was vortexed. After centrifuging, the supernatant phase was removed and sorbent particles containing the extracted analytes were eluted using acetonitrile. After that, this phase containing the analytes was taken and used in the following dispersive liquid – liquid microextraction process that was done to more enrichment the analytes using a deep eutectic solvent. After optimisation of the extraction process, sensitive limits of detection and quantification were found in the ranges of 0.21–0.28 and 0.70–0.93 ng g−1, respectively. Acceptable extraction recoveries of the analytes ranged from 60 to 73%. The method was selective regarding the analytes, as there were no interfering peaks eluted at the retention times of the analytes. After the validation step, the offered method was employed for the determination of the tetracycline antibiotics in various egg samples and the effect of ultraviolet waves in decontaminating them from egg samples was investigated.