Abstract
The AmpF LSTR NGM SElect™ is a next generation kit developed by Applied Biosystems that contains the 5 new loci specified in the recently expanded European Standard Set (ESS) together with the remaining markers from the SGM Plus ® kit, plus the highly discriminating SE33. We performed a validation study of the NGM SElect Kit with evaluation of critical parameters as species specificity, sensitivity, degradation/inhibition study, performance on a wide variety of forensic samples, mixture sample analyses. Our feedback confirmed the multiplex shows a robust PCR chemistry and the improved performance requested by the forensic community for typing difficult samples and offers the option of a 29 or 30 cycle protocol for optimum data quality and sensitivity.
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