VALIDATION OF A RAPID AND SIMPLE LC-MS/MS METHOD TO DETERMINE TRIMETAZIDINE IN RAT PLASMA

Abstract

A rapid, simple, and sensitive LC-MS/MS method was developed and validated for the determination of trimetazidine in rat plasma, using lidocaine as the internal standard (IS). The chromatographic separation was accomplished on a Atlantis dC18 column (150 × 2.1 mm, 5 µm) with the mobile phase consisting of acetonitrile and 10 mM ammonium formate (3:7, v/v) (pH 3.05, adjusted with formic acid). Plasma samples were deproteined with acetonitrile (1:3) and 3 μL of the supernatant was injected into the system. The retention times of trimetazidine and IS were approximately 1.45 min and 2.10 min, respectively. Calibration curves were linear over the concentration range of 0.5–500 ng/mL (1000 fold). The intra- and inter-batch precision, accuracy, and the relative standard deviation were all <15%. This method was successfully applied to determine trimetazidine concentrations in rat plasma.