Abstract
The Black Sea offers numerous harnessing possibilities for the medical and pharmaceutical, agricultural, food industry and cosmetic fields. Collagen extraction from the Black Sea fish is a research area of great interest. The purpose of this paper is to optimize the collagen quantitative analysis method based on hydroxyproline reagent through visible molecular absorption spectrometry. The adapted method was validated, achieving the following performance criteria: linearity, detection and quantification limits, accuracy/fidelity, stability/sturdiness, repeatability, and measurement uncertainty. The validated method was applied for the quantitative determination of collagen content in Grey Mullet fish and for the evaluation of collagen extraction output.
Highlights
The Black Sea offers numerous harnessing possibilities for the medical and pharmaceutical, agricultural, food industry and cosmetic fields
The validated method was applied for the quantitative determination of collagen content in Grey Mullet fish and for the evaluation of collagen extraction output
The sample to be analyzed is subjected to hydrolysis with sulphuric acid at 105°C, when collagen is transformed into hydroxyproline; the hydrolysate is filtered, diluted and the hydroxyproline is oxidized in the presence of chloramine T, and the oxidation product is decarboxylated to pyrrole; in the presence of pdimethylaminobenzaldehyde, a red colored compound is formed
Summary
The Black Sea offers numerous harnessing possibilities for the medical and pharmaceutical, agricultural, food industry and cosmetic fields. Samples containing collagen material are first subjected to acid hydrolysis, followed by the quantitative determination of hydroxyproline through high performance liquid chromatography or gas chromatography [18, 19], using volatile derivates, such as esters [20], butyl trifluoroacetate, isobutyl or N-heptafluorobutyryl [20, 21]. These are arduous methods that are more suitable for samples with a low content of collagen. The purpose of this paper is the optimization of the quantitative analysis method of collagen obtained from saltwater fish, based on the determination of hydroxyproline content through visible spectrophotometry molecular absorption. The validated method was applied for the quantitative determination of collagen content in Grey Mullet fish and for the evaluation of collagen extraction output
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