Abstract
The objective of this study was to estimate sensitivity (Se) and specificity (Sp) of a novel enzyme-linked immunosorbent assay (ELISA) test (using a coating antigen from Pasteurella multocida M-1404 via heat extraction) and an indirect hemagglutination (IHA) test for detection of Hemorrhagic septicemia (HS) in dairy cows, under Thai conditions, using a Bayesian approach. Dairy cow sera with a total of 1236 samples from 44 farms were tested with the two tests to detect immune responses against the HS. Percentages of positive samples for the ELISA and IHA tests were 73% (901/1236) and 70% (860/1236), respectively. Estimated sensitivity and estimated specificity of the ELISA test were 90.5% (95% posterior probability interval (PPI) = 83.2–95.4%) and 70.8% (95% PPI = 60.8–79.8%), respectively. Additionally, estimates for the Se and Sp values of the IHA test were 77.0% (95% PPI = 70.8–84.1%) and 51.1% (PPI = 36.8–66.3%), respectively. The estimated prevalence of the disease was 71.7% (95% PPI = 62.7–82.6%). These results demonstrate that the ELISA test can be a useful tool for the detection of the presence of an antibody against the HS in dairy cows. Notably, the cows in this area indicated a high percentage of exposure to Pasteurella multocida.
Highlights
Hemorrhagic septicemia is an acute, fatal and septicemic disease of buffaloes, beef, and dairy cows
These results demonstrate that the enzyme-linked immunosorbent assay (ELISA) test can be a useful tool for the detection of the presence of an antibody against the Hemorrhagic septicemia (HS) in dairy cows
The objective of this study was to estimate the performance of a novel indirect ELISA (using coating antigen from P. multocida M-1404 (B:2) via heat extraction) and indirect hemagglutination (IHA) tests among dairy cows in Thailand, using a Bayesian approach
Summary
Hemorrhagic septicemia is an acute, fatal and septicemic disease of buffaloes, beef, and dairy cows. The disease is mainly caused by Pasteurella multocida (P. multocida), which consists of specific serotypes, including B:2 (Asian serotype) and E:2 (African serotype). The pathogen is a gram-negative non-endospore forming bacteria and is known as a multiple killing agent [1]. Case fatality rates of relevant disease outbreaks may be above 80% [2]. It has been reported that mortality losses (direct losses) account for 92.7% of total losses, while the rest (7.3%) were due to treatment costs, growth reduction, and low milk yields [3]. Research in Thailand using an indirect enzyme-linked immunosorbent assay (ELISA) has reported that the seroprevalence of the disease in dairy cows was
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