Abstract
A liquid chromatographic tandem mass spectrometric method for the quantitative determination of sumatriptan base in human plasma and urine has been developed and validated over the concentration range 0.2–20 ng base ml −1. Sumatriptan is a 5-HT 1 receptor agonist which has found widespread use in the treatment of migraine. Sumatriptan and its internal standard (D 3-sumatriptan) were extracted from human matrices using C 2 solid phase cartridges. The extracts were chromatographed on a C 18 column, ionised using a heated nebuliser assisted atmospheric pressure ionisation (API) interface and detected by MS/MS in the multiple reaction monitoring mode. The completed validation demonstrated the method to be robust, accurate, precise and specific for the direct quantification of sumatriptan in human fluids. The method was used on a routine basis to determine the levels of sumatriptan in human volunteers following the oral administration of a 25 mg dose of sumatriptan succinate.
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