Abstract
An HPLC method with fluorescence detection suitable for routine determination of levofloxacin in plasma and dialysate has been validated. Sample preparation was assured by one-step protein precipitation for plasma or direct injection of the dialysate solution, respectively. Separation occurred on an YMC Pro C18 RP column (150 mm × 2 mm) with an acidic binary gradient mobile phase and detection at excitation and emission wavelengths of 296 and 504 nm. The assay was linear between 0.1 and 6 μg/ml for plasma and 0.1 and 5 μg/ml for dialysate with intra- and inter-day precision and accuracy lower than 10%. No degradation of levofloxacin was observed under the applied conditions for both matrices. The method was successfully applied to an in vitro pharmacokinetic study and patient samples as well.
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